cell culture huvecs  (PromoCell)

Journal: BMC Cancer

Article Title: Exploration of RNA-binding proteins identified RPS27 as a potential regulator associated with Kaposi’s sarcoma development

doi: 10.1186/s12885-025-13790-0

Figure Lengend Snippet: siRPS27 increased the pro-tumor signatures of HUVECs. A Bar plot showing the expression level of RPS27 mRNA in WT, siNC, and siRPS27 samples. B Western blot results show the expression level of RPS27 protein in three groups. The right panel shows the quantitative result. C Cell cycle results show the percentages of three cellular stages in three groups. The right panel shows the quantitative result. D Bar plot showing the cellular proliferation results in three groups. E Cell migration results show the migrated cell numbers in three groups. The right panel shows the quantitative result. F Cell invasion results show the invaded cell numbers in three groups. The right panel shows the quantitative result. G Tube formation results show the tube length in three groups. The right panel shows the quantitative result. * p -value < 0.05; ** p -value < 0.01; *** p -value < 0.001; Student’s t -test

Article Snippet: After culturing each group of cells in serum-free HUVEC cell-specific culture medium for 48 h, a cell suspension was prepared, and 50 μL of cell suspension (1 × 10 5 cells) was seeded in the plate coated with Matrigel and incubated for another 12 h. Representative photographs were subsequently captured under the microscope (IX53, OLYMPUS, Japan) after co-culture.

Techniques: Expressing, Western Blot, Migration